Fig. 4

Effect of TSH on the mRNA expression of ALP, COI1, BMP2 and Runx2. The mRNA expression of ALP (a), COI1 (b), BMP2 (c) and Runx2 (d) was determined by real-time RT-PCR. ALP (e) and BMP2 (f) mRNA levels were detected at 0, 12, 24 and 48 h of 100 mU/mL TSH treatment. The data are expressed as the means±SDs. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control group. As shown by western blot analysis, TSH (10 and 100 mU/mL) promoted Runx2 and osterix protein expression (g)